Bioactive natural products is an important area of research, and a large number of researchers are engaged in screening natural products (crude extracts of natural origin) for desired bioactivity(ies). This article discusses some of the issues usually encountered in this area of research, and how they can be tackled.
Natural Products, Antimicrobial Susceptibility Tests, Post-Extract Effect,Precipitation, Abiotic Controls
[1]
Houghton PJ, Raman A (1998). Analysis of crude extracts, fractions and isolated compounds. In: Laboratory handbook for the fractionation of natural extracts: Springer; p. 113-38.
[2]
Kothari Vijay, Gupta Ankit, Naraniwal Madhu (2012). Comparative study of various methods for extraction of antioxidant and antibacterial compounds from plant seeds. Journal of Natural Remedies, 12(2):162-173.
[3]
Pfaller MA, Sheehan DJ, Rex JH (2004). Determination of fungicidal activities against yeasts and molds: lessons learned from bactericidal testing and the need for standardization. Clinical Microbiology Review, 17(2):268.
[4]
Ramadan MA, Tawfik AF, Shibl AM, Gemmell CG (1995). Post-antibiotic effect of azithromycin and erythromycin on streptococcal susceptibility to phagocytosis. Journal of Medical Microbiology, 42:362-366.
[5]
Ramanuj Kruplai, Pooja Bachani, Vijay Kothari (2012). In vitro antimicrobial activity of certain plant products/seed extracts against multidrug resistant Propionibacterium acnes, Malassezia furfur, and aflatoxin producing Aspergillus flavus. Research in Pharmacy, 2(3): 22-31.
[6]
Shah P. M. (1982) Paradoxical effect of antibiotics I. The ‘Eagle effect’. Journal of Antimicrobial Chemotherapy, 10(4):259-60
[7]
Wadhwani T, Desai K, Patel D, Lawani D, Bahaley P, Joshi P, et al. (2009). Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials. Internet Journal of Microbiology 2009; 7(1).
